What is the product "Cryoprecipitated AHF Pooled"?

Pooled Cryoprecipitate is usually made up of 10 units of thawed cryoprecipite (cryo).   One unit of cryo can  increase the level of fibrinogen in an average-size adult by 5mg/dL.  The average level of fibrinogen for a normal adult is >100mg/dL.   Therefore, an effective dose usually consisted of pooling 10 units of cryo together.  So, when cryoprecipitate is ordered it is usually pooled into one bag which is then called   "Cryoprecipitated AHF Pooled".

Cryoprecipitated AHF Pooled can also be pooled prior to labeling, freezing, and storage. If pooled promptly after preparation using aseptic technique and refrozen immediately. The label should stated the number of units pooled.

Procedure for pooling cryoprecipitate:

1. Frozen cryo is thawed in a protective plastic overwrap in a waterbath at 30-37C up to 15 minutes. 

NOTE:  Do not refreeze after thawing.

Transfer Bag	Label the satellite transfer bag. Hemostat the tubing of the transfer bag. Open one of the ports on a unit of cryo to be pooled
	Spike the opening of the port with the transfer bag coupler (spike).
	Release the hemostat and allow the cryo to flow into the transfer bag.   Replace the hemostat and remove spike.  Repeat procedure until all units of cryo are in the transfer bag.

6. Remove as much air as possible form the transfer bag and heat seal the transfer bag tubing with the heat sealer and cut the tubing.
7. Weigh the content of the bag and record on label.

Storage and Expiration of Pooled CRYO:

• Pooled cryo is stored at room temperature 20-24^oC and transfused as soon as possible after thawing.
• Pooled cryo expires 4 hours after pooling.

Quality Control:

• Each pool must have a Factor VIII content of at least 80 IU units times the number of donor units in the pool.
• Each pool must also have Fibrinogen content of at least 150mg times the number of donor units in the pool.

Indications of usage:

• Hemophilia A
• Von Willebrand’s disease
• Factor XIII deficiency
• A source of fibrinogen for hypofibrinogenemia
• To make fibrin glue, a substance composed of cryoprecipitate (fibrinogen) and topical thrombin